Breeding initiatives of seeds of landraces, amateur varieties and conservation varieties : an inventory and case studies

One of the objectives of the Farm Seed Opportunities (FSO) project is to develop on farm breeding methodologies for the conservation and development of landraces, amateur and conservation varieties. The starting point for the development of these methodologies are the already existing experiences of farmers, small scale seed producers and researchers. To be able to draw on the expertise of these practitioners, we have selected five breeding initiatives for in depth case-studies. Prior to the selection of the five cases we carried out an inventory of all known initiatives within the European Economic Area. Results are presented in this repor

Varieties and seeds for organic farming: a solution for cabbage through participatory breeding in Brittany

In partnership with INRA, professionals from the organic vegetable sector in Brittany conducted a breeding programme for organically-farmed cabbage based on an evaluation programme of genetic resources collected from French (INRA) and European gene banks at the PAIS (Agro-biological platform of the Interprofessional Association for Organic Farming in Brittany), located in Suscinio. Between 2001 and 2003, the evaluation focused on quality criteria for commercial products and the suitability of varieties to the organic market, as well as on their adaptation to the environment. About 400 populations of cauliflower have thus been evaluated at the PAIS and/or by professionals in the sector in Brittany in order to evaluate their adaptation to organic production under different soil conditions and their suitability to various marketing channels. Ten of them were selected. Focused at first on cabbage, the programme was extended at the request of professionals and led to the breeding of several other species (tomato, carrot, lettuce, spinach, etc.). Breeding programs must now be optimized in order to satisfy consumer quality and adaptability requirements, and to design a coherent regulatory framework for participatory plant breeding and organic farming

A call for innovations tht change the (organic) world

Recent developments in organic farming and consumption show dynamic growth in markets; new uptake of Organic Agriculture by farmers, however, has been slow. Hindrances appear to be production problems or lack of trust by the farmers that organic methods can solve farming problems, such as fertilization, plant protection, animal health, efficient use of workforce, marketing diversity etc. While in some cases those problems can be solved through learning existing and regionally practiced methods, innovations are imperative to make organic farming competitive and a viable alternative farming system. OFIA, the Organic Farming Innovation Award, part of the Organic World Congress, highlights outstanding innovations and publishes priorities for innovative research

Sélection participative et adaptation locale

The use of organic seeds has been compulsory since 2005 for practicing organic farming. Despite the efforts made by seed companies, applications are not yet fully satisfied in quantity and diversity. Producers of Bio Loire Ocean made the bet to develop a research program on this issue. How to set up and select specific varieties for their territories and production systems? Choice was made to work with varieties populations because of their plasticity and diversity. With the help of INRA SAD, a local participatory breeding work was initiated on many vegetable crops including leading species in the region (carrot, salad) and other species for diversification (parsnips, tomatoes,...). The results are encouraging and the producers wish to pursue and develop specific selection on their farms in order to have organic varieties adapted to their pedoclimatic and socio-economic environments. Like the partnership established with AGROCAMPUS OUEST and the network Carotte et autres Daucus, we wish to promote the genetic diversity of crop varieties and meet the needs of producers and consumers. Our program will grow in the coming years, involving and combining the skills and expertise of researchers, producers and multipliers for an overall, coherent and sustainable organic vegetable seed industry in the region of Pays de Loire

Cultivating diversity and food quality. Proceedings of Diversifood EU Forum, Brussels, 11 April 2018

To tackle this issue, Diversifood team organised a forum with policy makers and stakeholders on the 11th of April 2018, in Brussels. Diversifood’s aim is to share results and key lessons including new approaches for the management of cultivated biodiversity, for plant breeding for sustainable farming systems, and new relationships among actors of food systems. In the afternoon, there was time for discussion, knowledge sharing, collecting feedback and extending current policies to include cultivating diversity and food quality (for FP9, CAP 2020, The outputs of this workshop will feed Diversifood’s final recommendations. The forum was kindly hosted by the European Committee of the Regions (Rue Belliard/Belliardstraat 101, 1040 Brussels)

Terroir is a key driver of seed-associated microbial assemblages

Seeds have evolved in association with diverse microbial assemblages that may influence plant growth and health. However, little is known about the composition of seed-associated microbial assemblages and the ecological processes shaping their structures. In this work, we monitored the relative influence of the host genotypes and terroir on the structure of the seed microbiota through metabarcoding analysis of different microbial assemblages associated to five different bean cultivars harvested in two distinct farms. Overall, few bacterial and fungal operational taxonomic units (OTUs) were conserved across all seed samples. The lack of shared OTUs between samples is explained by a significant effect of the farm site on the structure of microbial assemblage, which explained 12.2% and 39.7% of variance in bacterial and fungal diversity across samples. This site-specific effect is reflected by the significant enrichment of 70 OTUs in Brittany and 88 OTUs in Luxembourg that lead to differences in co-occurrence patterns. In contrast, variance in microbial assemblage structure was not explained by host genotype. Altogether, these results suggest that seed-associated microbial assemblage is determined by niche-based processes and that the terroir is a key driver of these selective forces

The organic seed regulations framework in Europe – current status and recommendations for future development

Organic agriculture regulations, in particular European regulation EC 889/2008, prescribe the use of organically produced seed. For many cultivated plants, however, organic seed is often not available. This is mainly because investment in organic plant breeding and seed production has been low in the past. To bridge the gap between organic seed supply and demand, national and European regulations define certain circumstances under which organic producers are permitted to use non-organically produced seed. While the organic sector currently depends on these concessions, they also threaten to impede a further increase in the demand for organic seed, thereby potentially restraining present and future investment in organic seed production and plant breeding. We review the current status of the organic seed regulations framework by analysing key issues such as the role of the national derogation regimes, the role of expert groups, databases and seed prices. Key points are that (a) the situation of the organic seed sector has improved over the last few years; however, (b) reporting on organic seed to the EU by different countries needs to be harmonised; (c) the success of the organic seed sector depends critically on the implementation and improvement of national expert groups; and (d) to protect genetic diversity, the use of local varieties and landraces should not be impeded by organic seed regulations

A Multi-Actor Literature Review on Alternative and Sustainable Food Systems for the Promotion of Cereal Biodiversity

Organic and low-input food systems are emerging worldwide in answer to the sustainability crisis of the conventional agri-food sector. “Alternative” systems are based on local, decentralized approaches to production and processing, regarding quality and health, and short supply-chains for products with strong local identities. Diversity is deeply embedded in these food systems, from the agrobiodiversity grown in farmers’ fields, which improves resilience and adaptation, to diverse approaches, contexts and actors in food manufacturing and marketing. Diversity thus becomes a cross-sectoral issue which acknowledges consumers’ demand for healthy products. In the framework of the European project “CERERE, CEreal REnaissance in Rural Europe: embedding diversity in organic and low-input food systems”, the paper aims at reviewing recent research on alternative and sustainable food systems by adopting an innovative and participatory multi-actor approach; this has involved ten practitioners and twenty-two researchers from across Europe and a variety of technical backgrounds in the paper and analysis stages. The participatory approach is the main innovation and distinctive feature of this literature review. Partners selected indeed what they perceived as most relevant in order to facilitate a transition towards more sustainable and diversity based cereal systems and food chains. This includes issues related to alternative food networks, formal and informal institutional settings, grass root initiatives, consumer involvement and, finally, knowledge exchange and sustainability. The review provides an overview of recent research that is relevant to CERERE partners as well as to anyone interested in alternative and sustainable food systems. The main objective of this paper was indeed to present a narrative of studies, which can form the foundation for future applied research to promote alternative methods of cereal production in Europe.Peer reviewe

The DEAD-box RNA Helicase DDX6 is Required for Efficient Encapsidation of a Retroviral Genome

Viruses have to encapsidate their own genomes during the assembly process. For most RNA viruses, there are sequences within the viral RNA and virion proteins needed for high efficiency of genome encapsidation. However, the roles of host proteins in this process are not understood. Here we find that the cellular DEAD-box RNA helicase DDX6 is required for efficient genome packaging of foamy virus, a spumaretrovirus. After infection, a significant amount of DDX6, normally concentrated in P bodies and stress granules, re-localizes to the pericentriolar site where viral RNAs and Gag capsid proteins are concentrated and capsids are assembled. Knockdown of DDX6 by siRNA leads to a decreased level of viral nucleic acids in extracellular particles, although viral protein expression, capsid assembly and release, and accumulation of viral RNA and Gag protein at the assembly site are little affected. DDX6 does not interact stably with Gag proteins nor is it incorporated into particles. However, we find that the ATPase/helicase motif of DDX6 is essential for viral replication. This suggests that the ATP hydrolysis and/or the RNA unwinding activities of DDX6 function in moderating the viral RNA conformation and/or viral RNA-Gag ribonucleoprotein complex in a transient manner to facilitate incorporation of the viral RNA into particles. These results reveal a unique role for a highly conserved cellular protein of RNA metabolism in specifically re-locating to the site of viral assembly for its function as a catalyst in retroviral RNA packaging

RNAi in the regulation of mammalian viral infections

Although RNA interference (RNAi) is known to play an important part in defense against viruses of invertebrates, its contribution to mammalian anti-viral defense has been a matter of dispute. This is surprising because all components of the RNAi machinery necessary for robust RNAi-mediated restriction of viruses are conserved in mammals, and the introduction of synthetic small interfering RNAs (siRNAs) into cells efficiently silences the replication of viruses that contain siRNA complementary sequences in those cells. Here, I discuss the reasons for the dispute, and review the evidence that RNAi is a part of the physiological defense of mammalian cells against viral infections